Can technical sequences be flexibly parsed, interpreted, and edited with splitcode?

Original title: Flexible parsing, interpretation, and editing of technical sequences with splitcode

Authors: Delaney K Sullivan,Lior Pachter

In this article, researchers introduce a new tool called splitcode that helps in analyzing and editing sequencing reads. Next-generation sequencing libraries often contain synthetic constructs like sequencing adapters, barcodes, and unique molecular identifiers, which are crucial for interpreting sequencing data. The splitcode program provides a flexible and efficient way to parse and interpret these sequences, making it easier to process reads from various types of sequencing assays. The tool is simple to use and ensures reproducibility in the preprocessing of reads from both single-cell and bulk sequencing experiments. The splitcode program is freely available for download on the GitHub website. The authors of the article have declared no competing interests.

Original article: https://www.biorxiv.org/content/10.1101/2023.03.20.533521v3